pCDH-CMV-mCherry-UBAP2L 1-600aa

LOCUS      pCDH-CMV-mCherry-UBAP2L 1-600aa            8952 bp DNA     circular SYN 21-AUG-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3240..5039
                     /codon_start=1
                     /label=UBAP2L 1-600AA
                     /translation="MMTSVGTNRARGNWEQPQNQNQTQHKQRPQATAEQIRLAQMISDH
                     NDADFEEKVKQLIDITGKNQDECVIALHDCNGDVNRAINVLLEGNPDTHSWEMVGKKKG
                     VSGQKDGGQTESNEEGKENRDRDRDYSRRRGGPPRRGRGASRGREFRGQENGLDGTKSG
                     GPSGRGTERGRRGRGRGRGGSGRRGGRFSAQGMGTFNPADYAEPANTDDNYGNSSGNTW
                     NNTGHFEPDDGTSAWRTATEEWGTEDWNEDLSETKIFTASNVSSVPLPAENVTITAGQR
                     IDLAVLLGKTPSTMENDSSNLDPSQAPSLAQPLVFSNSKQTAISQPASGNTFSHHSMVS
                     MLGKGFGDVGEAKGGSTTGSQFLEQFKTAQALAQLAAQHSQSGSTTTSSWDMGSTTQSP
                     SLVQYDLKNPSDSAVHSPFTKRQAFTPSSTMMEVFLQEKSPAVATSTAAPPPPSSPLPS
                     KSTSAPQMSPGSSDNQSSSPQPAQQKLKQQKKKASLTSKIPALAVEMPGSADISGLNLQ
                     FGALQFGSEPVLSDYESTPTTSASSSQAPSSLYTSTASESSSTISSNQSQESGYQSGPI
                     QSTTYTSQNNAQGPLYEQRSTQTR"
     misc_feature    complement(5004..5042)
                     /label=Primer R for UBAP2L 1-600AA
     misc_feature    5022..5060
                     /label=Primer F for UBAP2L 1-600AA
     misc_feature    5049..5636
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5520..5531)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5709..5942
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6014..6135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6154..6289
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6327..6343)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6351..6367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6375..6405)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6420..6441
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6729..7317)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7488..8348)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8349..8453)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8927..8943
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tgatgacatc ggtgggcact aaccgagccc ggggaaactg ggaacaacct caaaaccaaa
     3301 accagacaca gcacaagcag cggccacagg ccactgcaga acaaattaga cttgcacaga
     3361 tgatttcgga ccataatgat gctgactttg aggagaaggt gaaacaattg attgatatta
     3421 caggcaagaa ccaggatgaa tgtgtgattg ctttgcatga ctgcaatgga gatgtcaaca
     3481 gagctatcaa tgttcttctg gaaggaaacc cagacacgca ttcctgggag atggtcggga
     3541 agaagaaggg agtctcaggc cagaaggatg gtggccagac ggaatccaat gaggaaggca
     3601 aagaaaatcg agaccgggac agagactata gtcggcgacg tggtgggcca ccaagacggg
     3661 ggagaggtgc cagccgtgga cgagagtttc gaggtcagga aaatggattg gatggcacca
     3721 agagtggagg gccttctgga agaggaacag aaagaggcag aaggggccgt ggccgaggca
     3781 gaggtggctc tggtaggcga ggaggaaggt tttctgctca aggaatggga acctttaacc
     3841 cagctgatta tgcagagcca gccaatactg atgataacta tggcaatagc agcggcaata
     3901 cgtggaacaa cactggccac tttgaaccag atgatgggac gagtgcatgg aggactgcaa
     3961 cagaggagtg ggggactgaa gattggaatg aagatctttc tgagaccaag atcttcactg
     4021 cctctaatgt gtcttcagtg cctctgcctg cggagaatgt gacaatcact gctggtcaga
     4081 gaattgacct tgctgttctg ctggggaaga caccatctac aatggagaat gattcatcta
     4141 atctggatcc gtctcaggct ccttctctgg cccagcctct ggtgttcagt aattcgaagc
     4201 agactgccat atcacagcct gcttcaggga acacattttc tcatcacagt atggtgagca
     4261 tgttagggaa aggatttggt gatgtcggtg aagctaaagg cggcagtact acaggctccc
     4321 agttcttgga gcaattcaag actgcccaag ccctggctca gttggcagct cagcattctc
     4381 agtctggaag caccaccacc tcctcttggg acatgggctc gacgacacaa tccccatcac
     4441 tggtgcagta tgatttgaag aacccaagtg attcagcagt gcacagcccc tttacaaagc
     4501 gccaggcttt taccccatct tcaaccatga tggaggtgtt ccttcaggag aagtcacctg
     4561 cagtggctac ctccacagct gcacctccac ctccgtcttc tcctctgcca agcaaatcca
     4621 catcggctcc acagatgtcg cctggatctt cagacaacca gtcctctagc cctcagccgg
     4681 ctcagcagaa actgaaacag cagaagaaaa aagcctcctt gacttctaag attcctgctc
     4741 tggctgtgga gatgcctggc tcagcagata tctcagggct aaacctgcag tttggggcat
     4801 tgcagtttgg gtcagagcct gtcctttctg attatgagtc cacccccacc acgagcgcct
     4861 cttcaagcca ggctccaagt agcctgtata ccagcacggc cagtgaatca tcctctacaa
     4921 tttcatctaa ccagagtcag gagtctggtt atcagagcgg cccaattcag tcgacaacct
     4981 atacctccca aaataatgct cagggccctc tttatgaaca gagatccaca cagactcggt
     5041 agggatccaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5101 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     5161 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     5221 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5281 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5341 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5401 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     5461 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5521 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     5581 cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct ccccgccggt
     5641 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     5701 ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac
     5761 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5821 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5881 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5941 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     6001 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6061 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6121 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc
     6181 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     6241 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     6301 agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6361 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6421 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6481 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6541 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6601 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6661 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6721 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6781 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6841 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6901 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6961 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7021 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7081 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7141 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     7201 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7261 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7321 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7381 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7441 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7501 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7561 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7621 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7681 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7741 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7801 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7861 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7921 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7981 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8041 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8101 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8161 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8221 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8281 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8341 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8401 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8461 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     8521 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     8581 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     8641 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     8701 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     8761 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     8821 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     8881 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     8941 agtgccaagc tg
//